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The BioSeparations Lab
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Recent Publications

2018

Soto-Sierra L, Stoykova P, Nikolov ZL. Extraction and fractionation of microalgae-based protein products. Algal Research. 2018;36:175-192

Guo, Shengchun, et al. “Exploring the separation power of mixed‐modal resins for purification of recombinant osteopontin from clarified E. coli lysates.” Biotechnology progress (2018).

Kulkarni S, Nikolov Z. Process for selective extraction of pigments and functional proteins from Chlorella vulgaris. Algal Research. 2018;35:185-193.

Ravi, A.; Guo, S.; Rasala, B.; Tran, M.; Mayfield, S.; Nikolov, Z.L. Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. Int. J. Mol. Sci. 2018, 19, 585.

Marrone et al., 2018. Review of the harvesting and extraction program within the National Alliance for Advanced Biofuels and Bio products, Algal Research, in press, doi.org/10.1016/j.algal.2017.07.015

2014-16

Munjal, N., Kulkarni, S., Quinones, K., Tran, M., Mayfield, S. P., & Nikolov, Z. L. (2015). Evaluation of pretreatment methods for primary recovery and capture of an antibody fragment (αCD22scFv) from Chlamydomonas reinhardtii lysates.Algal Research, 12, 455-462.

Wilken, L. Ogle, E.T., Lohrman, T. and Nikolov, Z.L.  2016. Impact of dry-milled germ processing on aqueous protein and oil extraction, Food and Bioprocess Technology 9: 612-620.

Munjal, N., Garzon-Sanabria, A. J., Quinones, K. W., Gregory, J., & Nikolov, Z. L. (2014). Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas reinhardtii. Processes, 2(3), 625-638.

Hood, N.C., Hood, K.R.,Woodard, S.L., Wilken L.R, Nikolov, Z.L., Howard, J.A, Egelkrout, E and Hood, E. Purification and Characterization of Recombinant Cel7A from Maize Seed. 2014. Appl Biochem.Biotechol. 174: 2864-2874.

2012-13

Barros, G.O.F., Woodard, S.L., Wilken, L.R., White, S.G.,Damaj, M. B., Mirkov, T. E., and Z. L. Nikolov. 2013. Recovery of Bovine Lysozyme from Transgenic Sugarcane Stalks. Bioprocess Biosyst. Eng. doi.10.1007/s00449-012-0878.

Egelkrout, E., McGaughey, K., Keener, T., Ferleman, A., Woodard, S., Devaiah, S., Nikolov, Z. L. Hood, E., and Howard. 2013. Enhanced Expression Levels of Cellulase Enzymes Using Multiple Transcription Units. Bioenerg. Res. 6: 699-710.

Garzon-Sanabria, A.J., Ramirez-Caballero, S.S., Moss, F., and Z. L. Nikolov. 2013. Effect of algogenic organic matter (AOM) and sodium chloride on Nannochloropsis salina flocculation efficiency. Bioresource Technology, 143: 231-237.

Garzon-Sanabria A. J, Davis, R., and Z.L. Nikolov. 2012. Harvesting Nannochloris oculata by inorganic flocculation. Bioresource Technol., 118: 418-424.

Wilken, L.R. and Z.L. Nikolov. 2012. Recovery and purification of plant-made foreign proteins. Biotechnol. Advances. 30: 419-433.

Wilken, L.R. and Z.L. Nikolov. 2012. Downstream processing of transgenic plant systems: Protein recovery and purification strategies. In: Ma, S. and A. Wang. (eds). Molecular Farming in Plants: Recent Advances and Future Prospects. Springer, New York. p. 217-57.

 

Recent Book Chapters

Wilken, L. R, Sierra, L.S., Dixon, C. K., Nikolov, Z. L. 2018. Production of functional proteins and bioactive ingredients in microalgae in Encyclopedia of Agriculture, Food, and Biological Engineering.

Nikolov, Z. L., Regan, J. T., Dickey. L. F. and. Woodard, S. Production and Purification of Antibodies from Transgenic Plants. In Process Scale Purification of Antibodies.(Gottschalk, U.),  John Wiley & Sons, Hoboken, NJ, pp 631-653, 2nd edition.  doi: 10.1002/9781119126942.ch29

 

 

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